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nucleotide sequence analysis meaning in Chinese

核苷酸序列分析

Examples

  1. Sars virus nucleotide sequence analysis based on signal processing
    病毒的核酸序列的分析
  2. In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
    扩增产物连接到pgem - teasy载体中,转化大肠杆菌dh5菌株,筛选氨苄青霉素抗性菌落,提取质粒经酶切鉴定、 pcr分析以及确证性测序证明,所克隆的1500bp左右的片段含有完整的3abc基因,与国外参考序列相比,同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后,亚克隆3abc基因至原核表达载体ptriex - 4neo中,通过酶切鉴定、 pcr扩增以及序列分析,发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失,碰巧在3ab基因后形成一终止密码子,但3ab基因的阅读框架完整,选出含有3ab基因完整阅读框架的阳性克隆,用iptg诱导表达,收集菌液进行sds - page电泳、 westernblotting分析,结果表明, 3ab基因在大肠杆菌中成功表达,其表达产物为分子量33 . 5ku的融合蛋白,并能被口蹄疫病毒阳性血清识别。经薄层扫描分析,表达量占总蛋白量的26以上。
  3. Benzyl chloride were used for extracting genomic dna of aspergillus . niger 14 , about 1 . 5kb specific fragment was obtained from genomic dna of aspergillus . niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase , after ligated with pmd18 - tvector , transformated into e . colidh5a competent cell successfully . 3 . nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
    用氯化苄法提取了aspergillus . niger14 ~ #基因组dna ,根据genebank中已知的黑曲霉植酸酶基因序列设计出一对特异性引物(上游引物: 5 ataggcatcatgggcgtctc3下游引物: 5 cagctaagcaaaacactccgc3 ) ,采用pyrobest ~ ( tm ) dnapolymerase (高保真dna聚合酶) ,通过pcr方法从aspergillus . niger14 ~ #基因组dna中扩增出了预期的1 . 5kb左右的特异性产物,将其与pmd18 - tvector连接后,转化e . colidh5菌株的感受态细胞,经质粒抽提、酶切鉴定,确认该目的产物已得到成功克隆。

Related Words

  1. cylic nucleotides
  2. nucleotide triplet
  3. nucleotide diphosphatase
  4. n nucleotide
  5. nucleotide triphosphatase
  6. purin nucleotide
  7. nucleotide substitution
  8. fraudulent nucleotide
  9. nucleotide residue
  10. nucleotide analogue
  11. nucleotide residue
  12. nucleotide sequence
  13. nucleotide sequence homology
  14. nucleotide site directed muta-genesis
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